Abstract. Antisense oligonucleotides (ASOs) are used to selectively inhibit the translation of disease-associated genes via Ribonuclease H (RNaseH)-mediated cleavage or steric hindrance. They are being developed as a novel and promising class of drugs targeting a wide range of diseases.
This study reveals a mechanism, based on RNA structure, that mediates the function of PAS RNAs in gene regulation. The authors describe a role for the non-coding antisense transcripts produced at Since the discovery of antisense technology, there have been numerous advances both in the structure and properties of oligonucleotides used as therapeutic agents. Compared to that of whole DNA or RNA, today RNAi is achieved using siRNA, dsRNA (double-stranded RNA), and shRNA (short hairpin RNA). Characteristics of antisense RNAs. Antisense transcripts are not evenly distributed across the genome. Both ends of protein-coding genes have a propensity for natural antisense transcription 7, 11; specifically, antisense transcription is enriched 250 nucleotides upstream of the transcription start site (TSS) 8, 9 and 1.5 kilobases downstream of sense genes 8, 12.

Many genetic neurological diseases result from the dysfunction of single proteins. Genetic therapies aim to modify these disease-associated proteins by targeting the RNA and DNA precursors. This review provides a brief overview of the main types of genetic therapies, with a focus on antisense oligonucleotides (ASOs) and RNA interference (RNAi). We use examples of new genetic therapies for

The vcDNA is negative-sense single-stranded and forms DNA/RNA hybrid with viral RNA. Although synthetic antisense DNA oligonucleotides have been used in suppression of mRNA expression and

In their study, they attempted to discern whether the high throughput sequencing reads originated from the sense strand by second-strand synthesis (from the DNA polymerase) or the antisense strand by first-strand synthesis (from the reverse transcriptase) . Since both strands displayed a similar bias pattern, they concluded that the second VEpi.
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    • dna sense vs antisense